| Heat | Place | Forename | Surname | Club | Perf | Age Cat |
| 2 | 1 | Meghan | Beesley | Birchfield Harriers | 53.44 | SenW |
| 2 | 2 | Beth | Dobbin | Edinburgh Southern Harriers | 53.72 | SenW |
| 2 | 3 | Ella | Barrett | Chesterfield & District AC | 56.41 | SenW |
| 3 | 1 | Nessa | Millet | Loughborough Students AC | 57.17 | SenW |
| 3 | 2 | Natalie | Ainge | Cannock & Stafford AC | 57.48 | SenW |
| 2 | 5 | Rachael | Scott | Wigan & District AC | 58.03 | SenW |
| 2 | 6 | Hermione | Plumptre | Birchfield Harriers | 58.14 | SenW |
| 4 | 2 | Rebecca | Walkden | Wigan & District AC | 01:00.10 | SenW |
| 4 | 3 | Becky | Quirke | University of Sheffield | 01:00.74 | SenW |
| 2 | 1 | Nicholas | Atwell | Herne Hill Harriers | 48.99 | SenM |
| 2 | 2 | Elliot | Dunn | Birchfield Harriers | 49.18 | SenM |
| 2 | 3 | Matt | Chant | Andover AC | 49.24 | SenM |
| 3 | 1 | Dale | Garland | Guernsey Island AAC | 49.59 | SenM |
| 2 | 4 | Richard | Strachan | Victoria Park & Tower Hamlets AC | 50.19 | SenM |
| 3 | 2 | Harry | Russell | University of Sheffield | 50.32 | SenM |
| 3 | 3 | Theodore | Zake | Brunel University | 51.15 | SenM |
| 4 | 1 | Oliver | Monk | Cheltenham & County Harriers | 51.21 | SenM |
| 4 | 3 | Oliver | Dane | University of Sheffield | 51.25 | SenM |
| 3 | 5 | Ryan | Lowe | Dudley & Stourbridge Harriers | 51.74 | SenM |
| 2 | 5 | Reece | Middleton | Deeside AAC | 52.68 | SenM |
| 5 | 2 | Jonathan | Hughes | Leicester Coritanians AC | 53.20 | SenM |
| 7 | 1 | Ewan | Somerville | University of Sheffield | 53.38 | SenM |
| 6 | 3 | Gareth | Surman | Derby AC | 53.84 | SenM |
| 6 | 4 | Matthew | Graham | Manchester Harriers & AC | 54.77 | SenM |
| 9 | 1 | Jordan | Matthews | Durham University AC | 58.25 | SenM |
| 9 | 2 | Jonny | Lowe (Vet50) | Mansfield Harriers | 59.97 | SenM |
| 9 | 3 | Stephen | Terry-Short (Vet45) | Cannock & Stafford AC | 01:00.86 | SenM |
| 9 | 5 | Mick | Wall | Steel City Striders | 01:02.35 | SenM |
| 2 | 4 | Katrina | Simpson | Halesowen AC | 57.71 | U20W |
| 3 | 4 | Hannah | Malpass | Cannock & Stafford AC | 59.90 | U20W |
| 3 | 5 | Georgia | Walsh | Wigan & District AC | 01:00.24 | U20W |
| 3 | 6 | Chloe | Wilde | Cannock & Stafford AC | 01:01.55 | U20W |
| 5 | 1 | Emily | Davis | North Shields Poly | 01:01.74 | U20W |
| 5 | 2 | Holly | Horton | University of Sheffield | 01:03.25 | U20W |
| 4 | 6 | Ailis | MacFarlane | Burton AC | 01:07.21 | U20W |
| 4 | 2 | Bradley | Ellison | Rotherham Harriers & AC | 51.23 | U20M |
| 3 | 4 | John | Trotman | East Cheshire Harriers & Tameside AC | 51.27 | U20M |
| 4 | 4 | Peter | Curtis | Guernsey Island AAC | 51.53 | U20M |
| 4 | 5 | Robert | Tweedale | Kingston upon Hull AC | 53.12 | U20M |
| 5 | 5 | Jacob | Briscoe | Wigan & District AC | 54.70 | U20M |
| 6 | 5 | Jacob | Chastney | Kingston upon Hull AC | 54.98 | U20M |
| 5 | 6 | Alex | Nixon | Middlesborough AC (Mandale) | 55.66 | U20M |
| 8 | 3 | Scott | Ram | Amber Valley & Erewash AC | 57.18 | U20M |
| 8 | 5 | Jayden | Saberton | City of Sheffield & Dearne AC | 57.74 | U20M |
| 7 | 4 | Luke | Sayer | Newark AC | 57.98 | U20M |
| 4 | 1 | Morgan | Spink | Doncaster AC | 59.38 | U17W |
| 3 | 3 | Tia | Anderson | Durham City Harriers & AC | 59.45 | U17W |
| 4 | 4 | Isabella | Or Kam Fat | City of Sheffield & Dearne AC | 01:02.51 | U17W |
| 4 | 5 | Jasmine | Greenaway | Charnwood AC | 01:03.75 | U17W |
| 5 | 3 | Helen | Bourne | Derby AC | 01:04.78 | U17W |
| 5 | 4 | Lauren | Rayner | Wakefield District H&AC | 01:05.56 | U17W |
| 5 | 5 | Charlotte | Read | City of Sheffield & Dearne AC | 01:05.66 | U17W |
| 6 | 1 | Jamie | Clowes | City of Stoke AC | 51.20 | U17M |
| 5 | 1 | Oliver | Bolland | City of Sheffield & Dearne AC | 52.12 | U17M |
| 6 | 2 | Callum | Clydesdale | Charnwood AC | 53.13 | U17M |
| 5 | 3 | Alex | Cooper | Notts AC | 53.71 | U17M |
| 5 | 4 | Danny | Sullivan | Notts AC | 53.89 | U17M |
| 7 | 2 | Omar | Holness | Birchfield Harriers | 53.90 | U17M |
| 7 | 3 | Joseph | Thorne | Charnwood AC | 53.91 | U17M |
| 8 | 1 | Jacob | Calvert | Bexley AC | 56.21 | U17M |
| 8 | 2 | Jacob | Butterfield | Leeds City AC | 56.45 | U17M |
| 8 | 4 | Ethan | Harrison | Southport Waterloo AC | 57.36 | U17M |
| 9 | 4 | Thomas | Barber | Birmingham Running & Tri Club BRAT | 01:00.88 | U17M |
| 1 | 1 | Ashley | Nemits | Warrington AC | 01:00.08 | U15G |
| 1 | 2 | Flora | Miskin-Young | Wreake & Soar Valley AC | 01:03.22 | U15G |
| 1 | 3 | Isabella | Merritt | Horwich RMI Harriers | 01:03.52 | U15G |
| 1 | 4 | Neve | Evans | Charnwood AC | 01:04.44 | U15G |
| 1 | 5 | Alyssia | Jackson | Horwich RMI Harriers | 01:13.71 | U15G |
| 1 | 1 | Joel | Vernon | Hallamshire Harriers | 53.79 | U15B |
| 1 | 2 | Ben | Brinsdon | Birchfield Harriers | 53.85 | U15B |
| 1 | 3 | Travis | Anderson | Durham City Harriers & AC | 01:00.10 | U15B |
| 1 | 4 | Bailey | Stirzaker | Unattached | 01:10.05 | U15B |